Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences
| Autor: | Nuraizat Abidin, Tan Shu-Qing, Siti Izera Ismail, Muhammad Saiful Ahmad-Hamdani, Noor Wahida Ismail-Suhaimy, Mohd Termizi Yusof, Mansor Hakiman, Dzarifah Zulperi, Rohaya Ibrahim, Daljit Singh Karam |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phylogenetic tree biology Bronzing Pantoea Virulence Plant Science Amplicon biology.organism_classification 01 natural sciences law.invention Microbiology 03 medical and health sciences 030104 developmental biology law Phylogenetics GenBank Polymerase chain reaction 010606 plant biology & botany |
| Zdroj: | Journal of Plant Pathology. 102:193-199 |
| ISSN: | 2239-7264 1125-4653 |
| Popis: | Bronzing disease of jackfruit was first detected in plantation areas in Selangor and Pahang, Malaysia. The symptoms included reddish discoloration of the affected fruit pulp and rags, which could reduce the fruit quality and discourage purchasers. In this study, species-specific PCR amplification with primers encoded for cps and hrp virulence genes showed all bacterial strains isolated from infected jackfruits with bronzing symptoms, displaying a 1.1 kb and 0.9 kb amplicon. Phylogenetic analyses of the cps and hrp gene sequences clustered all strains to Pantoea stewartii subspecies stewartii (P. stewartii subsp. stewartii) reference strains (GenBank accession Nos. AF077292, AF282857, KY965964 and KY965965), with 62 to 80% similarity. Results revealed the presence of these virulence genes in P. stewartii subsp. stewartii strains is required for pathogenicity of the bacterium in jackfruit. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink