Structure-function relationships in the renal NaCl cotransporter (NCC)
| Autor: | Paola de los Heros, Antonio Vega-Mateos, Norma Vázquez, Christopher P. Cutler, Consuelo Plata, Gerardo Gamba, Erika Moreno |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Kidney biology urogenital system Reabsorption Xenopus 030204 cardiovascular system & hematology biology.organism_classification Amino acid Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry Membrane protein embryonic structures parasitic diseases medicine Distal convoluted tubule Receptor Cotransporter |
| DOI: | 10.1016/bs.ctm.2019.01.003 |
| Popis: | The thiazide-sensitive Na+-Cl- cotransporter (NCC) is the major pathway for salt reabsorption in the distal convoluted tubule, serves as a receptor for thiazide-type diuretics, and is involved in inherited diseases associated with abnormal blood pressure. The functional and structural characterization of NCC from different species has led us to gain insights into the structure-function relationships of the cotransporter. Here we present an overview of different studies that had described these properties. Additionally, we report the cloning and characterization of the NCC from the spiny dogfish (Squalus acanthias) kidney (sNCC). The purpose of the present study was to determine the main functional, pharmacological and regulatory properties of sNCC to make a direct comparison with other NCC orthologous. The sNCC cRNA encodes a 1033 amino acid membrane protein, when expressed in Xenopus oocytes, functions as a thiazide-sensitive Na-Cl cotransporter with NCC regulation and thiazide-inhibition properties similar to mammals, rather than to teleosts. However, the Km values for ion transport kinetics are significantly higher than those observed in the mammal species. In summary, we present a review on NCC structure-function relationships with the addition of the sNCC information in order to enrich the NCC cotransporter knowledge. |
| Databáze: | OpenAIRE |
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