Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles — An efficient and flexible novel tool for enzyme catalyzed biotransformations
| Autor: | Csaba László Bencze, Botond Nagy, Ferenc Ender, Csaba Paizs, László Poppe, Peter Palovics, Diána Weiser |
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| Rok vydání: | 2016 |
| Předmět: |
Fluid Flow and Transfer Processes
Green chemistry Chemistry Organic Chemistry Microfluidics Nanochemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Biotransformation Chemical engineering Chemistry (miscellaneous) Biocatalysis Magnetic nanoparticles Organic chemistry Liquid bubble 0210 nano-technology Layer (electronics) |
| Zdroj: | Journal of Flow Chemistry. 6:43-52 |
| ISSN: | 2063-0212 2062-249X |
| Popis: | Biotransformation of l-phenylalanine (l-1a) and five unnatural substrates (rac-1b–f) by phenylalanine ammonia-lyase (PAL) was investigated in a novel microfluidic device (Magne-Chip) that comprises microliter volume reaction cells filled with PAL-coated magnetic nanoparticles (MNPs). Experiments proved the excellent reproducibility of enzymecatalyzed biotransformation in the chip and the excellent reusability of the enzyme layer during 14 h continuous measurement (>98% over 7 repetitive measurements with l-1a). The platform also enabled fully automatic multiparameter measurements with a single biocatalyst loading of about 1 mg PAL-MNP. Computational fluid dynamics (CFD) calculations were used to study the flow field in the chambers and the effect of unintended bubble formation. Optimal flow rate for l-1a reaction and specific activities for rac-1b–f under these conditions were determined. |
| Databáze: | OpenAIRE |
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