Preparation of riboflavin specifically labeled in the 5′-hydroxymethyl terminus using a vitamin B2-aldehyde-forming enzyme from Schizophyllum commune
| Autor: | Dale E. Edmondson, Donald B. McCormick, Tea N. Kekelidze |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Organic Chemistry Schizophyllum commune Riboflavin biology.organism_classification Biochemistry Aldehyde Chemical synthesis Analytical Chemistry chemistry.chemical_compound Sodium borohydride chemistry Benzyl alcohol Drug Discovery Organic chemistry Phenol heterocyclic compounds Radiology Nuclear Medicine and imaging Hydroxymethyl Spectroscopy |
| Zdroj: | Journal of Labelled Compounds and Radiopharmaceuticals. 36:953-960 |
| ISSN: | 0362-4803 |
| DOI: | 10.1002/jlcr.2580361007 |
| Popis: | A method is described for synthesis of riboflavin selectively labeled in the hydrogens at the 5′-hydroxymethyl position. In this method, a vitamin B2-aldehyde-forming enzyme from Schizophyllum commune is used to specifically and completely oxidize the 5′-hydroxymethyl of riboflavin to the 5′-aldehyde. This reaction is monitored spectrophotometrically by the reduction of 2,6-dichlorophenolindophenol at 600 nm. Appearance of aldehyde product was directly quantitated by reverse-phase high-performance liquid chromatography. Product is extracted from the incubation mixture by phenol after saturation with (NH4)2SO4 and then further purified by benzyl alcohol extraction. The 5′-aldehyde is reduced with appropriately labeled sodium borohydride to yield the vitamin specifically labeled in the 5′-hydroxymethyl group. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text