Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma
Autor: | Veronika Medvecká, Anna Zahoranová, Stanislav Kyzek, Juliána Tomeková, Eliška Gálová |
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Rok vydání: | 2020 |
Předmět: |
010302 applied physics
Atmospheric pressure Chemistry Plasma parameters General Chemical Engineering Analytical chemistry food and beverages chemistry.chemical_element Atmospheric-pressure plasma General Chemistry Plasma Condensed Matter Physics 01 natural sciences Nitrogen Oxygen 010305 fluids & plasmas Surfaces Coatings and Films 0103 physical sciences Fourier transform infrared spectroscopy Water vapor |
Zdroj: | Plasma Chemistry and Plasma Processing. 40:1571-1584 |
ISSN: | 1572-8986 0272-4324 |
Popis: | Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (Pisum sativum L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air. |
Databáze: | OpenAIRE |
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