Microtubule nanospool formation by active self-assembly is not initiated by thermal activation
| Autor: | Ofer Idan, Henry Hess, Shruti B. Seshadri, Ashutosh Agarwal, Jasmine D. Crenshaw, Parag Katira, Takahiro Nitta, Matthew Downs, John Gonzales, Isaac Luria, Simon R. Phillpot, Shivendra Pandey, Jovan Kamcev |
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| Rok vydání: | 2011 |
| Předmět: |
Streptavidin
animal structures Materials science musculoskeletal neural and ocular physiology Brownian ratchet General Chemistry Condensed Matter Physics Crystallography chemistry.chemical_compound stomatognathic system chemistry Microtubule Biotinylation parasitic diseases Thermal Biophysics Kinesin Self-assembly |
| Zdroj: | Soft Matter. 7:3108-3115 |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/c0sm00802h |
| Popis: | Biotinylated microtubules partially coated with streptavidin and gliding on a surface coated with kinesin motors can cross-link with each other and assemble into nanospools with a diameter of a few micrometres. The size distribution of these nanospools is determined, and it is shown with simulations of microtubule gliding that these spools are too small to be formed by thermally activated turns in the gliding direction (a Brownian ratchet mechanism). Instead, spool formation is primarily the result of two processes: pinning of gliding microtubules to inactive motors and simultaneous cross-linking of multiple microtubules. |
| Databáze: | OpenAIRE |
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