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When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoR1, a DNA fragment of the size of 14.0 kb can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSV src ). The cellular DNA fragment contains the entire M-MSV src specific sequences. The 14.0 kb EcoRl DNA fragment was cloned in bacteriophage λ. The sequence organization of a recombinant clone, λ·M TX -1, was analyzed by restriction endonuclease mapping, S1 mapping and electronmicroscopy. The results indicate that λ·M TX -1 contains an uninterrupted stretch of 1.0 kb similar to that found in the M-MSV genome. |
