Popis: |
Thin-layer chromatographic-enzymatic identification and the mode of action of chlorinated hydrocarbon insecticides 1. It is shown that chlorinated hydrocarbons influence the activity of bovine liver esterase. 2. Most of the compounds inhibit the enzyme, but DDT, DDD, DDE, Perthane, and methoxychlor activate it. 3. After UV-irradiation on thin-layer plates all compounds show strong inhibition activity against bovine liver esterase. 4. Almost all of the chlorinated hydrocarbons studied are decomposed by UV-irradiation as was shown by their R F -values obtained from thin-layer chromatography on Silica Gel G. Although DDT inhibits bovine liver esterase after UV-irradiation its insecticidal activity is lost, and it is supposed that this effect is due to inability of the new compound to penetrate the insect. |