Use of Yeast Plasmids: Transformation and Inheritance Assays
| Autor: | Joyce S. K. Chew, Anastasiia Mereshchuk, Melanie J. Dobson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetics
0303 health sciences 030306 microbiology Saccharomyces cerevisiae Inheritance (genetic algorithm) Biology medicine.disease_cause biology.organism_classification Yeast 03 medical and health sciences Transformation (genetics) Plasmid Shuttle vector medicine Escherichia coli Gene 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071608678 |
| DOI: | 10.1007/978-1-0716-0868-5_1 |
| Popis: | The use of the budding yeast Saccharomyces cerevisiae as a model genetic organism has been facilitated by the availability of a wide range of yeast shuttle vectors, plasmids that can be propagated in Escherichia coli and also in yeast, where they are stably maintained at low- or high-copy number, depending on the plasmid system. Here we provide an introduction to the low-copy (ARS/CEN) and multi-copy (2-μm-based) plasmids, the marker genes commonly used for plasmid selection in yeast, methods for transforming yeast and monitoring plasmid inheritance, and tips for working with yeast transformants. |
| Databáze: | OpenAIRE |
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