Rapid Determination of Montelukast in Human Plasma by LC-ESI-MS/MS and Its Application to a Bioequivalence Study

Autor:	Gunta Subbaiah, Hiten J. Shah, N. K. Patel, Mohan L. Kundlik, Mallika Sanyal, Pranav S. Shrivastav
Rok vydání:	2009
Předmět:	Electrospray Chemical ionization Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Mass spectrometry Biochemistry Analytical Chemistry Human plasma Electrochemistry medicine Protein precipitation Sample preparation Quantitative analysis (chemistry) Spectroscopy Montelukast medicine.drug
Zdroj:	Analytical Letters. 42:2041-2059
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032710903082721
Popis:	A rapid liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS) method was developed for the determination of montelukast in human plasma. The extraction of montelukast from plasma (300 µL) involved protein precipitation. Quantitation was performed using LC-ESI-MS/MS, operating in the positive ion and selective reaction monitoring (SRM) mode. The total chromatographic run time for the analysis was 1.5 min. A linear dynamic range was established from 5 to 800 ng mL−1 for montelukast. The method was fully validated especially with regard to real subject sample analysis. It was successfully applied to a bioequivalence study in 18 healthy human subjects under fed condition (human subjects were allowed to eat as per the prescribed diet for bioequivalence study).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7208a8ebcb7f04ba9fc43c49cf78328c https://doi.org/10.1080/00032710903082721 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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