Enhanced in vitro developmental competence of sheep embryos following sericin supplementation of the in vitro maturation and in vitro culture media
| Autor: | F. Aghaz, H. Karami Shabankareh, Hadi Hajarian |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
030219 obstetrics & reproductive medicine urogenital system Chemistry Embryogenesis 0402 animal and dairy science Semen Embryo culture Embryo 04 agricultural and veterinary sciences Oocyte 040201 dairy & animal science Sericin In vitro maturation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Endocrinology Food Animals Internal medicine embryonic structures medicine Animal Science and Zoology Blastocyst |
| Zdroj: | Small Ruminant Research. 136:257-260 |
| ISSN: | 0921-4488 |
| DOI: | 10.1016/j.smallrumres.2016.01.019 |
| Popis: | The objective was to determine the effects of various sericin concentrations (0, 0.1, 0.5, 1, and 2.5%) during in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on ovine embryo development. Pubertal Sanjabi ewes’ oocytes were aspirated from slaughterhouse derived ovaries and matured in vitro. Semen was collected from a healthy fertile ram, capacitated and the in vitro matured oocytes were fertilized using capacitated spermatozoa. The IVM and IVC media were supplemented with 0, 0.1, 0.5, 1 and 2.5% of sericin. Addition of 0.5% of sericin during IVM and IVC significantly increased cleavage and blastocyst rates (85.48 ± 1.57 and 50.07 ± 3.04, respectively) compared with 0 and 0.1% of sericin. However, a too high concentration (2.5%) of sericin during IVM and IVC decreased cleavage and blastocyst rates compared with low concentrations (P < 0.05). In conclusion, appropriate concentrations (0.1 and 0.5%) of sericin promoted ovine blastocyst formation in vitro, although a high concentration of sericin suppressed embryo development. |
| Databáze: | OpenAIRE |
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