Somatic embryogenesis of the heavy metal accumulator Prosopis laevigata

Autor: Francisco Cruz-Sosa, J. Orozco-Villafuerte, Eduardo J. Vernon-Carter, L. Buendía-González, M. E. Estrada-Zúñiga
Rok vydání: 2011
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 108:287-296
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-011-0042-4
Popis: Somatic embryogenesis and whole plant regeneration was achieved in callus cultures derived from immature zygotic embryos of Prosopis laevigata (Humb. & Bonpl. ex Willd.) M.C. Johnst., recently identified as chromium (Cr), cadmium (Cd), lead (Pb) and nickel (Ni) accumulator. Embryogenic calli were induced on Murashige and Skoog (MS) medium added with a mixture of organic components plus N-6 benzyladenine (BA) (6.62 μM) and 2,4-dichlorophenoxyacetic acid (2,4-D) (2.26 μM) or thidiazuron (4.54–9.08 μM) and indole-3-acetic acid (1.42 μM). Embryogenic calli transferred onto half-strength MS medium without plant growth regulators developed globular embryos, of which 20% matured when treated with 3.75% (w/v) polyethylene glycol (PEG), and of these 50% fully differentiated into plantlet embryo. Regenerated plants were successfully acclimatized (90%), while in vitro seedlings transferred to MS medium containing 0.5 mM Cd, Cr, Ni or Pb, exhibited high heavy metals accumulation (627 mg Cr kg−1, 5,688 mg Cd kg−1, 1,148 mg Ni kg−1, and 3,037 mg Pb kg−1 dry weight) and efficient roots to shoots translocation (42–73%).
Databáze: OpenAIRE
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