A Specific Progesterone Receptor in the Hamster Uterus: Physiologic Properties and Regulation During the Estrous Cycle1
| Autor: | B. W. O′Malley, Wendell W. Leavitt, D. O. Toft, C. A. Strott |
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| Rok vydání: | 1974 |
| Předmět: | |
| Zdroj: | Endocrinology. 94:1041-1053 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-94-4-1041 |
| Popis: | Our previous studies indicated the presence of an estrogen-dependent, progesteronebinding system in the hamster uterus. A progesterone- binding component has now been characterized in the uterine cytosol fraction by density gradient centrifugation (6–7S sedimentation coefficient in sucrose-glycerol gradients), binding equilibrium measurements (KA = 1.7 ± 0.08 × 109) M−1) and competition assays. The hormone specificity of the progesterone-binding component was indicated by the relative binding activity of progesterone (100), deoxycorticosterone (64) and 5α-pregnanedione (20). Other hormonal steroids tested or progesterone metabolites did not compete effectively for :3H-progesterone-binding sites. The concentration of progesterone-binding sites in uterine cytosol was calculated from Scatchard plot analysis of data obtained by a dextran-charcoal adsorption method. During the estrous cycle, fluctuations in the concentration of progesteronebinding sites on a wet weight, cytosol protein or DNA basis were correl... |
| Databáze: | OpenAIRE |
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