Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI
| Autor: | Rainer Kaufmann, Vojtěch Pražák, Kay Grünewald |
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| Rok vydání: | 2021 |
| Předmět: |
Correlative
0303 health sciences business.industry Cryo-electron microscopy technology industry and agriculture virus diseases Electron Biology Laser Fluorescence law.invention 03 medical and health sciences Optics law parasitic diseases Microscopy Fluorescence microscope natural sciences Electron microscope business 030304 developmental biology |
| Zdroj: | Methods in Cell Biology ISBN: 9780128220580 |
| Popis: | The combination of super-resolution fluorescence microscopy and electron microscopy at ambient temperatures has become an established technique and a broad variety of modalities are now available to the cell biology community. In contrast, correlative cryogenic super-resolution fluorescence and electron microscopy (super-resolution cryo-CLEM) is just emerging. Aside from technical challenges, one of the major issues is the risk of devitrification of the specimen caused by the laser intensities required for super-resolution imaging. Cryo-SOFI (cryogenic super-resolution optical fluctuation imaging) allows the reconstruction of super-resolution images at particularly low laser intensities. It is fully compatible with the standard sample preparation for cryogenic electron microscopy (cryo-EM) and fairly easy to implement in any standard cryogenic fluorescence microscope. |
| Databáze: | OpenAIRE |
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