| Autor: |
Y Mouton, Pierre Wattré, Gunnar V. Alm, Didier Hober, Georges Lion, V Chieux, Wassim Chehadeh, Jeanne Harvey |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology. 49:660-666 |
| ISSN: |
0300-9475 |
| DOI: |
10.1046/j.1365-3083.1999.00552.x |
| Popis: |
We investigated the biological properties of interferon (IFN)-alpha produced by Sendai virus (SV)-activated whole blood cultures in 20 patients infected with human immunodeficiency virus (HIV)-1 and 24 healthy controls. Supernatants of cultures were assayed for IFN-alpha by using an immunological method (DELFIA), biological methods and an in-vitro MxA induction assay. The levels of intracellular MxA protein were detected by an immunochemiluminescence assay. The levels of IFN-alpha in patients measured by DELFIA were significantly lower than those in healthy controls (P < 0.0001), but the antiviral activity of IFN-alpha in patients infected with HIV-1 was lower than predicted from DELFIA. The IFN-alpha produced by cells of patients infected with HIV-1 was able to induce MxA protein in human amnions WISH cells but was unable to protect these cells against Vesicular Stomatitis Virus (VSV)-induced cytopathic effects. A relative increased capability to induce the production of MxA protein in vitro was observed with the IFN-alpha contained in culture supernatant of virus-activated whole blood of HIV-1-infected patients with increased levels of MxA in their peripheral blood. These data suggest that biological properties of IFN-alpha produced in the course of HIV-1 infection are different from those observed with IFN-alpha of healthy subjects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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