Analytical study of extracts of St John’s wort (Hypericum perforatum), evaluation of HPTLC plates by multivariate data analysis

Autor: Gudrun Roos, Urs Simmen, Karl-Artur Kovar, Kim Wuthold
Rok vydání: 2003
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 16:15-18
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.16.2003.1.3
Popis: HPTLC with multivariate data analysis has been used as a new means of evaluating the quality of herbal extracts. Spectral data from three-dimensional chromatograms obtained from St John’s wort ( Hypericum perforatum ) extracts were correlated with opioid receptor affinity to predict pharmacological activity. A total of 27 extracts of St John’s wort from four different accessions extracted with seven different solvents (100, 80, 60, 40 and 20% EtOH in water and 90 and 60% MeOH in water) were analyzed at λ = 200 to 600 nm by use of a diode-array scanner. The correlation between the spectroscopic data and IC 50 values obtained from non-selective opioid binding to rat cortex was determined by means of a quantitative model using a partial least-squares algorithm (PLS-1); the correlation coefficient was 0.97. The model was then verified by prediction of the IC 50 values of seven other extracts of St John’s wort. Spectroscopic HPTLC analysis combined with multivariate data analysis was shown to be suitable for c...
Databáze: OpenAIRE
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