Prenatal exposure to hexavalent chromium disrupts testicular steroidogenic pathway in peripubertal F1 rats
| Autor: | Sakhila K. Banu, Felicia Mary Michael, Ajit Kumar Navin, Shobana Navaneethabalakrishnan, Mariajoseph Michael Aruldhas, N. Srinivasan, Kathireshkumar Mani |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
0303 health sciences medicine.medical_specialty biology medicine.drug_class Chemistry Steroidogenic acute regulatory protein 010501 environmental sciences Toxicology Androgen Sertoli cell 01 natural sciences 03 medical and health sciences medicine.anatomical_structure Endocrinology Estrogen Hormone receptor CYP17A1 Internal medicine medicine biology.protein Aromatase Receptor 030304 developmental biology 0105 earth and related environmental sciences |
| Zdroj: | Reproductive Toxicology. 101:63-73 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2021.01.014 |
| Popis: | We have reported sub-fertility in F1 progeny rats with gestational exposure to hexavalent chromium [Cr(VI)], which had disrupted Sertoli cell (SC) structure and function, and decreased testosterone (T). However, the underlying mechanism for reduced T remains to be understood. We tested the hypothesis "transient prenatal exposure to Cr(VI) affects testicular steroidogenesis by altering hormone receptors and steroidogenic enzyme proteins in Leydig cells (LCs)." Pregnant Wistar rats were given drinking water containing 50, 100, and 200 mg/L potassium dichromate during gestational days 9-14, encompassing fetal differentiation window of the testis from the bipotential gonad. F1 male rats were euthanized on postnatal day 60 (peripubertal rats with adult-type LCs alone). Results showed that prenatal exposure to Cr(VI): (i) increased accumulation of Cr(III) in the testis of F1 rats; (ii) increased serum levels of luteinizing and follicle stimulating hormones (LH and FSH), and 17β estradiol, and decreased prolactin and T; (iii) decreased steroidogenic acute regulatory protein, cytochrome P450 11A1, cytochrome P450 17A1, 3β- and 17β-hydroxysteroid dehydrogenases, cytochrome P450 aromatase and 5α reductase proteins, (iv) decreased specific activities of 3β and 17β hydroxysteroid dehydrogenases; (v) decreased receptors of LH, androgen and estrogen in LCs; (vi) decreased 5α reductase and receptor proteins of FSH, androgen, and estrogen in SCs. The current study concludes that prenatal exposure to Cr(VI) disrupts testicular steroidogenesis in F1 progeny by repressing hormone receptors and key proteins of the steroidogenic pathway in LCs and SCs. |
| Databáze: | OpenAIRE |
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