An efficient rinsing procedure for 10 mm cell culture inserts prior to coculture studies

Autor: Michael V. Dodson, Janet L. Vierck, Elizabeth A. Krabbenhoft, John H. Howard
Rok vydání: 1996
Předmět:
Zdroj: Methods in Cell Science. 18:25-29
ISSN: 1573-0603
1381-5741
DOI: 10.1007/bf00123520
Popis: A goal of our laboaatory is to define putative intercellular communication mechanisms between myofibers and adipocytes. In pursuit of this objective we established a co-culture system incorporating commercially available microporous cell culture inserts. These cell culture inserts provide separation of cells bathed in a common media environment. Isolation and evaluation of the conditioned media from such systems allows detection of autocrine or paracrine agent production by either cell type. However, the process of rinsing cells plated on inserts prior to initiating co-culture may damage insert integrity and may dilute (added) defined treatment medium by introducing residual rinse medium. We have developed a method to rinse microporous membrane inserts before the addition of defined treatment medium. This rinsing technique decreases damage to inserts and reduces the volume of residual medium on all physical aspects of the insert.
Databáze: OpenAIRE
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