Chromosomes of the Lancelet Branchiostoma belcheri Gray

Autor:	Kyoko Saotome, Yoshio Ojima
Rok vydání:	2001
Předmět:	Chromosome number biology Lancelet Anatomy biology.organism_classification Molecular biology chemistry.chemical_compound chemistry Branchiostoma belcheri Sodium citrate Glass slide Colchicine Animal Science and Zoology Ploidy
Zdroj:	Zoological Science. 18:683-686
ISSN:	0289-0003
DOI:	10.2108/zsj.18.683
Popis:	We characterized the chromosomes of the lancelet Branchiostoma belcheri Gray. Testes were removed from adult males, cut up, treated with colchicine (25–50 μg/ml) for 3–6 hr, and dissociated into single cells by pippeting. The dissociated cells were swollen with 5.0–6.5% sodium citrate for 10 min and fixed with methanol-acetic acid (3:1). The fixed cells were dropped onto a glass slide and air-dried. The haploid chromosome number was 18 and diploid number was 36.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::719e9aa41d8e1022c1d58ac74879f04e https://doi.org/10.2108/zsj.18.683 Zobrazit plný text záznamu Plný text ve formátu PDF