Role of reactive oxygen metabolites in aspirin-induced gastric damage in humans: gastroprotection by vitamin C

Autor:	Thomas Brzozowski, Stanislaw J. Konturek, Wolfram Domschke, André Markmann, Andrzej Moniczewski, I. R. Van Der Voort, Jan C. Becker, Jan W. Konturek, Thorsten Pohle
Rok vydání:	2001
Předmět:	chemistry.chemical_classification medicine.medical_specialty Antioxidant Hepatology biology medicine.medical_treatment Glutathione peroxidase Gastroenterology Glutathione medicine.disease_cause digestive system diseases Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Endocrinology chemistry Internal medicine Myeloperoxidase medicine biology.protein Pharmacology (medical) Oxidative stress Peroxidase
Zdroj:	Alimentary Pharmacology & Therapeutics. 15:677-687
ISSN:	0269-2813
Popis:	Background: The roles of active oxygen metabolites and anti-oxidative defenses in aspirin (ASA)-induced gastric damage have been little studied. Aim: We determined the effects of aspirin (400 mg b.d.) with or without vitamin C (480 mg b.d.) for 3 days on gastric mucosa in human volunteers. Methods: Gastric injury was assessed endoscopically; gastric blood flow, reactive oxygen release (quantified by chemiluminescence), lipid peroxidation, myeloperoxidase, superoxide dismutase and glutathione peroxidase activity and intragastric vitamin C content were measured. Expression of superoxide dismutase and glutathione peroxidase mRNAs was assayed semi-quantitatively. Results: ASA produced erosions, a marked increase in chemiluminescence, lipid peroxidation, and myeloperoxidase activity. It also resulted in a suppression of gastric blood flow, intragastric vitamin C levels, superoxide dismutase and glutathione peroxidase activities. The addition of vitamin C significantly attenuated gastric damage and reversed the effects of ASA on these parameters. Superoxide dismutase and glutathione peroxidase mRNAs were decreased in ASA-treated subjects; the addition of vitamin C restored their regular levels. Conclusions: (i) free radical-induced lipid peroxidation and suppression of antioxidizing enzymes play an important role in gastric damage induced by aspirin; (ii) increased myeloperoxidase activity suggests activated neutrophils to be the major source of these radicals; (iii) vitamin C protects against ASA-induced damage due to its anti-oxidizing activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::719b9b46f00a27cee22a37d847f60b2c https://doi.org/10.1046/j.1365-2036.2001.00975.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.