Salt regulation of O-acetylserine(thiol)lyase in Arabidopsis thaliana and increased tolerance in yeast

Autor:	José R. Domínguez-Solís, Cecilia Gotor, Luis C. Romero, Gloria Gutiérrez-Alcalá
Rok vydání:	2001
Předmět:	chemistry.chemical_classification biology Physiology Plant Science Cysteine synthase biology.organism_classification Lyase Yeast chemistry.chemical_compound chemistry Biochemistry Plant protein Genetics biology.protein Thiol Arabidopsis thaliana O-Acetylserine Cysteine
Zdroj:	Plant Physiology and Biochemistry. 39:643-647
ISSN:	0981-9428
DOI:	10.1016/s0981-9428(01)01277-3
Popis:	The cytosolic isoform of O -acetylserine(thiol)lyase (OASTL, EC 4.2.99.8), the enzyme that catalyses cysteine biosynthesis, is induced in leaves of Arabidopsis thaliana exposed to salt stress. The protein level increases after 1 d sodium chloride treatment, and progressively for up to 5 d. The overexpression in yeast of the Atcys-3A gene coding for this OASTL isoform can support the growth of the yeast cells at high concentrations of sodium chloride, suggesting that the plant protein is able to confer salt tolerance in yeast. Besides, the O -acetylserine(thiol)lyase enzyme activity is not inhibited in vitro by cations such as Li + or Na + . Our results suggest that the plant is responding to the salt stress by inducing cysteine biosynthesis as a protection against high ion concentrations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::718a97fccc911170a740959ab3862ed0 https://doi.org/10.1016/s0981-9428(01)01277-3 Zobrazit plný text záznamu Full Text from ScienceDirect