Effect of gamma irradiation of Moringa oleifera seed lectin on its larvicidal, ovicidal, and oviposition-stimulant activities against Aedes aegypti

Autor: Daniela Maria do Amaral Ferraz Navarro, Ana Patrícia Silva de Oliveira, C.A. Benevides, Lidiane Pereira de Albuquerque, Thiago Henrique Napoleão, Emmanuel Viana Pontual, Patrícia Maria Guedes Paiva, L.C.B.B. Coelho, Nataly Diniz de Lima Santos
Rok vydání: 2020
Předmět:
Zdroj: South African Journal of Botany. 129:3-8
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2018.05.001
Popis: The water-soluble Moringa oleifera lectin (WSMoL) exhibits larvicidal, ovicidal, and oviposition-stimulant effects on Aedes aegypti. Here, we evaluated the influence of gamma irradiation on the activity of WSMoL against A. aegypti. Irradiation with 10 mGy and 10 Gy did not result in the loss of protein molecules; however, irradiated samples presented a distinct profile on gel filtration chromatography. Bis-ANS probe fluorescence was similar when incubated with non-irradiated and 10 mGy-irradiated samples, but less intense fluorescence was observed when incubated with 10 Gy-irradiated WSMoL, indicating the exposure of lectin hydrophobic pockets to water. Irradiation of WSMoL with 10 mGy potentiated its larvicidal and ovicidal activities, while irradiated WSMoL with 10 Gy did not affect the survival of larvae and eggs. The stimulation of oviposition by WSMoL was not observed with irradiated samples. Nevertheless, the hatching rate of eggs laid on vessels containing 10 mGy-irradiated lectin (37%) was lower than that in vessels containing non-irradiated WSMoL (59%). In conclusion, a low dose of gamma irradiation (10 mGy) can be used to improve the deleterious effects of WSMoL on A. aegypti larvae and eggs, while a higher dose (10 Gy) was found to impair the insecticidal effects on this mosquito. These changes are probably linked to conformational changes in the structure of WSMoL.
Databáze: OpenAIRE
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