Two RP-HPLC assay methods with different chromatographic approaches for the simultaneous estimation of bambuterol and its main degradation product, terbutaline
| Autor: | Mohamed I. Walash, Samah Abo El Abass Mohamed |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Aqueous solution Chemistry General Chemical Engineering 010401 analytical chemistry Terbutaline General Engineering 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography Dosage form 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Column chromatography medicine Particle size Bambuterol 0210 nano-technology Triethylamine medicine.drug |
| Zdroj: | Analytical Methods. 11:1680-1688 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c9ay00123a |
| Popis: | The present study describes two stability-indicating HPLC methods with two different chromatographic approaches for the simultaneous estimation of bambuterol and its main degradation product, terbutaline in bulk and pharmaceuticals. Method I was carried out using a conventional particle-packed cyano column (150 mm × 4.6 mm, 5 μm particle size) and a micellar eluent composed of 0.1 M SDS, 15% n-propanol, and 0.3% triethylamine (pH 3.5), coupled with UV detection at 220 nm. Method II was based on the fused core shell technology, and the separation was carried out using a Kinetex C18 column (150 mm × 4.6 mm, 5 μm particle size) and an aqueous mobile phase composed of methanol and a 0.1 M phosphate buffer (45 : 55 v/v; pH 4), using fluorescence detection at λex = 275 nm and λem = 313 nm. The first method was rectilinear over the concentration range of 10–200 μg mL−1 for bambuterol and 5–100 μg mL−1 for terbutaline while the second method showed a good linearity in the range of 1–5 μg mL−1 for bambuterol and 0.1–1 μg mL−1 for terbutaline. The developed methods enabled the quantification of bambuterol and terbutaline in their dosage forms, including tablets and syrups and showed good results compared to the official methods. The practical and analytical comparisons between the two proposed methods is discussed. |
| Databáze: | OpenAIRE |
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