Effect of cannabinoids on glycine-activated currents in pyramidal neurons of the rat hippocampus

Autor:	Natalia Yatsenko, N. A. Lozovaya
Rok vydání:	2007
Předmět:	Cannabinoid receptor Physiology G protein General Neuroscience medicine.medical_treatment Anandamide Endocannabinoid system chemistry.chemical_compound Electrophysiology chemistry Biochemistry Biophysics medicine lipids (amino acids peptides and proteins) Cannabinoid Receptor Glycine receptor
Zdroj:	Neurophysiology. 39:13-19
ISSN:	1573-9007 0090-2977
DOI:	10.1007/s11062-007-0003-z
Popis:	Using electrophysiological techniques (a patch-clamp technique in the whole-cell configuration and intracellular perfusion of neurons), we studied the effect of cannabinoids on the characteristics of glycine-activated currents in freshly isolated pyramidal neurons of the rat hippocampus. We found that endocannabinoids (anandamide and 2-arachidonoyl glycerol), as well as a synthetic cannabinoid, WIN 55,212-2, when applied in physiological concentrations, decreased the amplitude of glycine-activated currents. The agents under study accelerated the kinetics of activation and desensitization of glycine-induced Cl− currents. The characteristics of the currents recovered after washout from cannabinoids. Changes in the kinetics of desensitization of glycine-activated currents depended noticeably on the holding potential; at positive potentials the sensitivity to cannabinoids was higher. These effects of cannabinoids were also observed in the presence of antagonists of CB1/CB3 receptors and an inhibitor of G proteins, GDPβS. These data indicate that under our experimental conditions cannabinoids exerted direct effects on glycine receptors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7179cf5183e10f2db2281d6108da5d00 https://doi.org/10.1007/s11062-007-0003-z Zobrazit plný text záznamu Full text from SpringerLink