| Autor: |
G. Gabay, H. Wang, J. Zhang, J. I. Moriconi, G. F. Burguener, T. Howell, A. Lukaszewski, B. Staskawicz, M.-J. Cho, J. Tanaka, T. Fahima, H. Ke, K. Dehesh, G.-L. Zhang, J.-Y. Gou, M. Hamberg, G. Santa Maria, J. Dubcovsky |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.08.25.505338 |
| Popis: |
Wheat is an essential crop for global food security and is well adapted to a wide variety of soils1. However, the gene networks regulating different root architectures remain poorly understood. We report here the identification of a cluster of a monocot-specific 12-OXOPHYTODIENOATE REDUCTASE genes from subfamily III (OPRIII) that modulate key differences in wheat root architecture associated with grain yield under water-limited conditions. Wheat plants with a loss-of-function mutation in OPRIII showed longer seminal roots, whereas plants with increased OPRIII dosage or transgenic over-expression showed reduced seminal root growth, precocious development of lateral roots and increased jasmonic acid (JA). A JA-biosynthesis inhibitor eliminated the root differences, confirming a JA-mediated mechanism. Multiple transcriptome analysis of transgenic and wild-type lines revealed significant enriched JA-biosynthetic and reactive oxygen species (ROS) pathways that paralleled changes in ROS distribution. The OPRIII genes provide a useful entry point to engineer root architecture in wheat and other cereals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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