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A Gram-stain-negative, aerobiotic, pink, motile, rod-shaped bacterium, designated P51 T was isolated from saline silt samples in Yantai, China. The novel isolate was able to grow at 4–42 °C (optimum 33 °C), pH 4.0–9.0 (optimum 7.0) and with 0–11.0% NaCl (optimum 4.0%, w/v). It could grow at 4 °C, which was different from the neighbour strains. The draft genome consisted of 4111 genes with a total length of 5139782 bp and 39.9% G + C content. The 16S rRNA gene sequence analysis indicated that strain P51 T was a member of the genus Echinicola and most closely related to ‘ Echinicola shivajiensis ’ with pair-wise sequence similarity of 97.7%. Genome analysis identified genes encoding proteins relating to carbon source utilization such as glycoside hydrolases and glycosyl transferases; carotenoid biosynthesis pathway and β -lactam resistance pathway were observed. Strain P51 T shared average nucleotide identity value below 84.7%, average amino acid identity value between of 70.8 – 89.3%, digital DNA-DNA hybridization identity of between 17.9–28.2% with the closely related type strains within the genus Echinicola . The sole menaquinone was MK-7; the major fatty acids were iso-C 15:0 , summed feature 3(C 16:1 ω 7 c and/or C 16:1 ω 6 c ), summed feature 4 (anteiso-C 17:1 B and/or iso-C 17:1 I) and summed feature 9 (iso-C 17:1 ω 9 c and/or 10-methyl C 16:0 ); the polar lipids included one phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, three unidentified aminolipids and one unknown lipid. Based on phenotypic, chemotaxonomic, and phylogenetic analyses, strain P51 T represents a novel species of the genus Echinicola , for which the name Echinicola salinicaeni sp. nov. is proposed. The type strain is P51 T (KCTC 82513 T = MCCC 1K04413 T ). |
