Aldehyde bearing bis-cyclometalated Ir(III) complex as selective photoluminescence turn-on probe for imaging intracellular homocysteine
| Autor: | Honglan Qi, Hongfang Gao, Chengxiao Zhang, Ying Zhao, Zhao Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Analytical chemistry
Quantum yield 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Aldehyde Adduct chemistry.chemical_compound Materials Chemistry Electrical and Electronic Engineering Instrumentation chemistry.chemical_classification Detection limit Ligand Metals and Alloys Buffer solution 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry 0210 nano-technology Luminescence Cysteine |
| Zdroj: | Sensors and Actuators B: Chemical. 245:853-859 |
| ISSN: | 0925-4005 |
| Popis: | A new photoluminescence (PL) turn-on probe based on aldehyde bearing bis-cyclometalated Ir(III) complex, [Ir(bt)2phen-CHO]+PF6− (1), is synthesized and applied to imaging homocysteine (Hcy) in living cells. The probe 1 is readily prepared by using 2-phenylbenzothiazole (bt) as main ligand and 1,10-phenanthroline-5-carboxaldehyde (phen-CHO) as ancillary ligand. Probe 1 displayed excellent selective PL enhancement in response to Hcy in neutral buffer solution based on aldehyde group cyclization reaction. The increased PL intensity of probe 1 is directly proportional to the concentration of Hcy in the range of 10 μM to 500 μM with a detection limit of 4.6 μM. More importantly, probe 1 can highly discriminate Hcy from cysteine (Cys), which is agreed with the higher luminescent quantum yield of the adduct of probe 1 and Hcy (1-Hcy, 0.46) compared with that of probe 1 and Cys (1-Cys, 0.05) in 10 mM phosphate buffer solution (pH 7.4). Furthermore, the probe 1 has appreciable cell permeability and low cytotoxicity and has been successfully applied to selectively imaging Hcy in living cells. The superior properties of the probe 1 make it of great potential use for studying the effects of Hcy in other biosystems. |
| Databáze: | OpenAIRE |
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