Influence of Chronic Lead Intoxication on Functional Characteristics and Isoform Composition of Left Ventricular Myosin in the Rat Heart

Autor:	S. R. Nabiev, L.V. Nikitina, Oksana P. Gerzen
Rok vydání:	2021
Předmět:	Gene isoform medicine.medical_specialty Physiology Chemistry Cooperativity macromolecular substances Isometric exercise Biochemistry In vitro Protein filament Endocrinology Lead acetate Internal medicine Myosin medicine Ecology Evolution Behavior and Systematics Actin
Zdroj:	Journal of Evolutionary Biochemistry and Physiology. 57:896-903
ISSN:	1608-3202 0022-0930
Popis:	The chronic lead intoxication was modelled by injecting outbred rats intraperitoneally with lead acetate 3 times a week for 5 weeks. Using an in vitro motility assay, it was shown that lead intoxication causes changes in in vitro actin–myosin interaction, specifically, a decrease in the maximum sliding velocity of native thin filaments over myosin isolated from the left ventricular myocardium of the rat heart. No statistically significant changes were found in the calcium sensitivity and cooperativity of the “pCa–velocity” curve, characteristics of the motile filament fraction, and isometric force. Using electrophoretic separation of proteins, a shift in the ratio of myosin heavy chains toward β-chains with a lower ATPase activity was found.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::713ba8277f5b03427217c702f1353ac8 https://doi.org/10.1134/s002209302104013x Zobrazit plný text záznamu Plný text ve formátu PDF