Design and optimization of biocompatible polycaprolactone/poly (l -lactic-co-glycolic acid) scaffolds with and without microgrooves for tissue engineering applications
| Autor: | Giovanna Ramos Garcez, Natália Fontana Nicoletti, Ricardo Meurer Papaléo, Jefferson Braga Silva, Nara Regina de Souza Basso, Pedro Cesar Chagastelles, Bruna Sgarioni, Cristhiane Alvim Valente, Fábio Herrmann, Maria M. Campos, Mara Lise Zanini, Gustavo Guthmann Pesenatto, Eduardo Goldani |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
0206 medical engineering technology industry and agriculture Metals and Alloys Biomedical Engineering Biomaterial macromolecular substances 02 engineering and technology Adhesion 021001 nanoscience & nanotechnology 020601 biomedical engineering Biomaterials Contact angle PLGA chemistry.chemical_compound Membrane chemistry Tissue engineering Polycaprolactone Ceramics and Composites 0210 nano-technology Cell adhesion Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. 106:1522-1534 |
| ISSN: | 1549-3296 |
| Popis: | This study investigated the effects of smooth and microgrooved membrane blends, with different polycaprolactone (PCL)/ poly(lactic-co-glycolic acid) (PLGA) ratios on the viability, proliferation, and adhesion of different mammalian cell types. The polymer matrices with and without microgrooves, obtained by solvent casting, were characterized by field-emission scanning electron microscopy, atomic force microscopy, contact angle and Young's modulus. Blend characterization showed an increase in roughness and stiffness of membranes with 30% PLGA, without any effect on the contact angle value. Pure PCL significantly decreased the viability of Vero, HaCaT, RAW 264.7, and human fetal lung and gingival fibroblast cells, whereas addition of increasing concentrations of PLGA led to a reduced cytotoxicity. Increased proliferation rates were observed for all cell lines. Fibroblasts adhered efficiently to smooth membranes of the PCL70/PLGA30 blend and pure PLGA, compared to pure PCL and silicone. Microgrooved membranes promoted similar cell adhesion for all groups. Microstructured membranes (15 and 20-μm wide grooves) promoted suitable orientation of fibroblasts in both PCL70/PLGA30 and pure PLGA, as compared to pure PCL. Neuronal cells of the dorsal root ganglion exhibited an oriented adhesion to all the tested microgrooved membranes. Data suggest a satisfactory safety profile for the microgrooved PCL70/PLGA30 blend, pointing out this polymer combination as a promising biomaterial for peripheral nerve regeneration when cell orientation is required. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1522-1534, 2018. |
| Databáze: | OpenAIRE |
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