Mechanisms of glucocorticoid uptake by isolated hepatocytes of the trout

Autor:	Brahim Lahlou, Jacqueline Porthé-Nibelle
Rok vydání:	1981
Předmět:	endocrine system medicine.medical_specialty Cell membrane permeability Physiology General Medicine Biology biology.organism_classification Biochemistry Trout Endocrinology Membrane Low affinity Internal medicine medicine Cortisone Salmo Molecular Biology Dexamethasone Glucocorticoid medicine.drug
Zdroj:	Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 69:425-433
ISSN:	0305-0491
DOI:	10.1016/0305-0491(81)90332-1
Popis:	1. 1. The cellular uptake of [3H]cortisol has been studied on hepatocytes isolated from the trout Salmo gairdneri irideus. 2. 2. Analysis of the uptake as a function of concentration indicated the presence of a low affinity saturable system (Km=60 μM) and a diffusion component. The saturable part involves metabolic energy and participation of membrane SH-groups. 3. 3. Uptake of [3H]cortisol was decreased when cells were incubated with a large excess of unlabelled cortisol or cortisone given simultaneously. 4. 4. Dexamethasone is much more permeant than cortisol (12 times) and displays an unusual “permissive” effect on cell membrane permeability to steroids. 5. 5. These results suggest that the uptake of cortisol by trout hepatocytes is mediated in part by a carrier protein located in the cellular membrane.
Databáze:	OpenAIRE
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