Radioimmunoassay of lysergic acid diethylamide (LSD) in serum and urine by using antisera of different specificities

Autor:	W. A. Ratcliffe, W. A. Harland, T E Levitt, S M Fletcher, A C Moffat, J. G. Ratcliffe
Rok vydání:	1977
Předmět:	Hallucinogen Antiserum Chromatography biology medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Serum albumin Radioimmunoassay Urine Blood serum Biochemistry Immunoassay biology.protein medicine Lysergic acid diethylamide medicine.drug
Zdroj:	Clinical Chemistry. 23:169-174
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/23.2.169
Popis:	We raised high-titre antisera to two LSD-bovine serum albumin conjugates, one linked via the indole nitrogen, the other via the amide side-chain. The antisera were specific for different parts of the LSD molecule, as demonstrated by cross-reactivity studies with LSD, its metabolites, ergot alkoloids, and closely related compounds. The antisera were used to develop a double-antibody radioimmunoassay with a detection limit of about 0.4 mug of LSD per liter of unextracted urine or serum. We saw no nonspecific interference by urine, serum, or from a series of commonly used drugs. There was good correlation between immunoassay values obtained with the two antisera (r = 0.91). However, the antiserum linked via the indole nitrogen gave consistently higher results for samples from persons who had taken LSD, owing to greater cross-reactivity with LSD metabolites. Radioimmunoassay by use of two such antisera is a more specific screening procedure for LSD abuse than has been available previously. In addition, antisera cross-reacting with LSD metabolites allow measurement of these compounds, for which there is no satisfactory method at the concentrations found in biological fluids in man.
Databáze:	OpenAIRE
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