| Popis: |
// Honglin Luo 1, 2, * , Huizan Yang 1, 3, * , Yong Lin 1, * , Yongde Zhang 1 , Chuanyan Pan 1 , Pengfei Feng 1 , Yanling Yu 1 and Xiaohan Chen 1 1 Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Institute of Fishery Sciences, Nanning, P.R. China 2 Guangxi Medical University, Nanning, P.R. China 3 College of Animal Science and Technology, Guangxi University, Nanning, P.R. China * These authors have contributed equally to this work Correspondence to: Honglin Luo, email: 541365548@qq.com Xiaohan Chen, email: Chnxhn@163.com Keywords: long non-coding RNA; oreochromis niloticus ; macrophages; HSP70; Streptococcus agalactiae Received: June 22, 2017 Accepted: August 17, 2017 Published: September 30, 2017 ABSTRACT Objectives: To investigate the lncRNA profiling during tilapia peritoneal macrophages (TPMs) activation and discuss the relationship between lncRNA and mRNA. Materials and Methods: RNA sequencing was used to investigate the lncRNA and mRNA profiles of TPMs activation following stimulation with Streptococcus agalactiae (Sa) antigen, heat shock protein 70 (HSP70) and HSP70+Sa. The expressions of lncRNA and mRNA were confirmed by qPCR. 356 lncRNA, 10173 mRNA and 1782 transcripts of uncertain coding potential (TUCP) were differentially expressed by pairwise comparison. These lncRNAs were shorter in length, fewer in exon number and higher in expression levels as compared with mRNAs. 683 lncRNAs and 4320 mRNAs were co-located, while 316 lncRNAs and 9997 mRNAs were in co-expression networks. Seven mRNAs (ANKRD34A, FMODA, GJA3, CNTN5, BMP10, BAI2 and HS3ST6) were involved in both networks of LNC_00035 and LNC_000466. Differentially expressed genes were involved in signaling pathways, such as “phosphorylation”, “cytokine-cytokine receptor interaction”, “endocytosis” and “MHC protein complex”. LNC_000792, LNC_000215, LNC_000035 and LNC_000310, with cis and/or trans relationships with mRNAs, were also involved in ceRNA network. Conclusions: These results might represent the first identified expression profile of lncRNAs and mRNAs in tilapia macrophages activated by HSP70 and Sa. |
