miR-330-3p promotes lung cancer cells invasion, migration, and metastasis by directly targeting hSOD2b
| Autor: | Fang Bai, Shanze Yi, Lianghua Shen, Feng Wang, Sijia Lei, Alexander Czachor, Hanxiao Sun, Mason Breitzig, Qing Zheng, Luyuan Huang, Shuaiguang Li |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Biomedical Engineering Bioengineering Biology 01 natural sciences Applied Microbiology and Biotechnology Metastasis 03 medical and health sciences In vivo 010608 biotechnology Drug Discovery microRNA medicine Lung cancer 030304 developmental biology 0303 health sciences Gene knockdown Transition (genetics) Process Chemistry and Technology General Medicine medicine.disease Manganese Superoxide Dismutase In vitro Cancer research Molecular Medicine Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 66:21-32 |
| ISSN: | 0885-4513 |
| Popis: | Lung cancer is a serious threat to human health. Studies have revealed that human manganese superoxide dismutase (hSOD2) and miRNAs play an essential role in the metastasis process of lung cancer. However, the miRNAs that associated with hSOD2 and involved in metastasis, remain elusive. After databases analysis and dual luciferase reporter validation, we demonstrated that miR-330-3p expression inversely correlated with hSOD2b expression level, and that miR-330-3p directly targeted the 3'untranslated region (3'UTR) of hSOD2b. Furthermore, overexpression of miR-330-3p promoted whereas knockdown of miR-330-3p inhibited invasion/migration and the epithelial-mesenchymal transition (EMT) process of lung cancer cells in vitro. Knockdown of miR-330-3p inhibited metastasis of lung cancer cells in vivo. Moreover, miR-330-3p-mediated enhancement of invasion/migration in 95-D cells could be rescued by over-expression of hSOD2. In conclusion, we demonstrated that miR-330-3p promoted metastasis of lung cancer cells by suppressing hSOD2b expression and unveiled a new clinical application of miR-330-3p in the therapy of lung cancer. |
| Databáze: | OpenAIRE |
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