Monoclonal antibody-based immunodot for evaluating serum immunoglobulin levels in rohu (Labeo rohita)

Autor:	Kalkuli Mariappa Shankar, Purandara Ballyaya Abhiman, Padinhate Purayil Suresh Babu, Badami Ramalingappa Honnananda
Rok vydání:	2014
Předmět:	biology Immunoglobulin levels medicine.drug_class Aquatic Science biology.organism_classification Monoclonal antibody law.invention Microbiology Labeo Titer Probiotic Immune system Antibody response law Immunology biology.protein medicine Antibody Agronomy and Crop Science
Zdroj:	Aquaculture International. 22:1451-1456
ISSN:	1573-143X 0967-6120
Popis:	Immune response in rohu treated with an immunomodulator is usually evaluated employing either non-specific immune parameters or traditional antibody-based tools. In the present study, a monoclonal antibody-based immunodot has been developed for evaluating antibody titre in rohu as a preliminary tool to ensure antibody response due to the effect of an immunomodulator, which can be used for routine field level analysis. The immunodot was sensitive enough to determine rohu immunoglobulin up to 15 μg/ml. Application of the immunodot for evaluating enhancement in immune response could be successfully demonstrated in probiotic fed rohu.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::70d3e47d8a72fb396d9028eaaebeecc6 https://doi.org/10.1007/s10499-014-9758-6 Zobrazit plný text záznamu Full text from SpringerLink