| Autor: |
Barreto de Albuquerque, Juliana, Altenburger, Lukas M, Abe, Jun, von Werdt, Diego, Wissmann, Stefanie, Martínez Magdaleno, Jose, Francisco, David, van Geest, Geert, Ficht, Xenia, Iannacone, Matteo, Bruggmann, Remy, Mueller, Christoph, Stein, Jens V |
| Rok vydání: |
2022 |
| Předmět: |
|
| DOI: |
10.48350/170765 |
| Popis: |
The gastrointestinal (GI) tract constitutes an essential barrier against ingested microbes, including potential pathogens. Although immune reactions are well studied in the lower GI tract, it remains unclear how adaptive immune responses are initiated during microbial challenge of the oral mucosa (OM), the primary site of microbial encounter in the upper GI tract. Here, we identify mandibular lymph nodes (mandLNs) as sentinel lymphoid organs that intercept ingested Listeria monocytogenes (Lm). Oral Lm uptake led to local activation and release of antigen-specific CD8+ T cells that constituted most of the early circulating effector T cell (TEFF) pool. MandLN-primed TEFF disseminated to lymphoid organs, lung, and OM and contributed substantially to rapid elimination of target cells. In contrast to CD8+ TEFF generated in mesenteric LN (MLN) during intragastric infection, mandLN-primed TEFF lacked a gut-seeking phenotype, which correlated with low expression of enzymes required for gut-homing imprinting by mandLN stromal and dendritic cells. Accordingly, mandLN-primed TEFF decreased Lm burden in spleen but not MLN after intestinal infection. Our findings extend the concept of regional specialization of immune responses along the length of the GI tract, with CD8+ TEFF generated in the upper GI tract displaying homing profiles that differ from those imprinted by lymphoid tissue of the lower GI tract. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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