Antibody-Antigen Equilibria in a Field of Magnetic Forces: Design of Reagentless Biosensors

Autor:	Cary J. Miller, Marcin Majda, Eliot Bennion, Shabnam Khoie, Laura N. Driscoll, Christopher F. Monson
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Analyte chemistry.chemical_compound Cardiac troponin chemistry Biomolecule Biophysics Antibody antigen Double stranded Biosensor DNA Microsphere
Zdroj:	ECS Transactions. 19:79-91
ISSN:	1938-6737 1938-5862
DOI:	10.1149/1.3118540
Popis:	This project concerns a new strategy of detecting biological molecules that relies on competitive exchange interactions of an analyte with two-component molecular tethers attaching superparamagnetic microspheres (4 micron in diameter) to a sensor surface. The individual tethers consist of either double stranded DNA or antibody-antigen complex and are designed to selectively detect either specific ssDNA or antigenic proteins, respectively, in sensitive reagentless fashion. Performance of sensors was investigated using human cardiac troponin I, a serum heart attack marker, as an example of analytes of credible relevance to biomedical diagnostics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::70c4cb29172a81fba91267d837f7ae94 https://doi.org/10.1149/1.3118540 Zobrazit plný text záznamu