| Autor: |
Hugo L. David, Veronique Levy-Frebault, Cécile P. Asselineau, Mamadou Daffé, Marie-Antoinette Lanéelle |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Annales de l'Institut Pasteur / Microbiologie. 134:241-256 |
| ISSN: |
0769-2609 |
| DOI: |
10.1016/s0769-2609(83)80037-4 |
| Popis: |
Summary A simple and quantitative method for the alkaline hydrolysis of fattyacid derivatives occurring in the lipids of mycobacteria is described. After methylation, the lipidic mixtures were chromatographed on a thin layer of silicagel. The contents in mycolates and secondary alcohols allowed the distinction of 9 groups among the 27 species studied. Such an analysis is generally insufficient to identify a species, but its discriminating power differs from that of other commonly-used methods. Complementary tests chosen according to each particular situation are necessary, including vapour phase chromatography applied to the same lipidic mixture as that used for thin-layer chromatography. Coupling of the two chromatographic methods would allow the recognition of 22 groups among the 27 species of mycobacteria studied. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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