Transgenic tomato expressing dsRNA of juvenile hormone acid O-methyl transferase gene of Helicoverpa armigera (Lepidoptera: Noctuidae) affects larval growth and its development
| Autor: | J Latha, Ramasamy Asokan, G. Sharath Chandra, N. K. Krishna Kumar, Manamohan Maligeppagol, M Navale Prakash, V. Krishna, K. Prasad Babu, R. Ellango |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Larva biology media_common.quotation_subject fungi food and beverages Helicoverpa armigera biology.organism_classification 01 natural sciences Microbiology Lepidoptera genitalia 010602 entomology 03 medical and health sciences 030104 developmental biology RNA interference Insect Science Juvenile hormone Botany Noctuidae Genetically modified tomato Metamorphosis media_common |
| Zdroj: | Journal of Asia-Pacific Entomology. 20:559-567 |
| ISSN: | 1226-8615 |
| DOI: | 10.1016/j.aspen.2017.03.016 |
| Popis: | Helicoverpa armigera is an important pest infesting a number of crops of high commercial value leading to huge economic losses globally. RNA interference (RNAi) is a potent tool for the control of insect pests and towards this objective, transgenic tomato expressing dsRNA of Juvenile hormone acid methyl transferase (JHAMT) gene of H. armigera was developed. The H. armigera larvae fed on HaJHAMT tomato leaves led to a severe (90%) down regulation of the cognate gene expression thus adversely affected the feeding and metamorphosis. Reduction in larval and pupal weight and inability to undergo complete metamorphosis were observed that led to larval-pupal intermediates and subsequently affected the eclosion. Thus, JH down regulation is an attractive target due to specificity to insects and its important role in insect growth and development to engineer insect resistance in crops using RNAi. |
| Databáze: | OpenAIRE |
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