| Autor: |
Hideto Tsuji, Masako Hamada, Surya Giri, T. Hagihara, T. Yamada, Hiroyuki Daimon, Misaki Kurobe, J. Harada |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings. |
| ISSN: |
0094-243X |
| DOI: |
10.1063/1.5089454 |
| Popis: |
This study aimed to isolate lactate-degrading bacteria via treatment with poly(L-lactic acid) (PLLA) in a thermophilic anaerobic digestion reactor to investigate their ecophysiological characteristics. Enrichment cultures were established with a basal medium (pH 7.0) supplemented with lactate and a thermophilic digester sludge as an inoculum, followed by anaerobic culturing at 55°C without shaking. Thereafter, 16S rRNA gene-based cloning analyses revealed that the predominant bacteria in the enrichment culture were of genus Moorella. Roll-tube cultures containing lactate and Moorella-resistant streptomycin were serially diluted to isolate Moorella cells from a series of the diluted enrichment cultures. Fluorescence in situ hybridization analyses using a Morl93 probe revealed that a pure culture of the strain (strain Hama-1) was successfully obtained. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Hama-1 is closely related to Moorella perchloratireducens strain An10T with 99.6% sequence similarity. Strain Hama-1 completely converted lactate to acetate, and was clearly phenotypically different from M. perchloratireducens strain An10T. Characterization of strain Hama-1 would further help the current understanding of lactate degradation to stably operate the thermophilic anaerobic digestion reactor using PLLA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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