Development and physico-chemical characterization of cyclodextrin–DNA complexes loaded liposomes

Autor:	Jorge L. Pesquero, Cristiane M. Viana, Mônica Cristina de Oliveira, Guilherme Diniz Tavares, Margareth Spangler Andrade, José G. V. C. Araújo, Wânia da Silva Carvalho, Gilson Andrade Ramaldes, José Mário Carneiro Vilela
Rok vydání:	2006
Předmět:	Mean diameter chemistry.chemical_classification Liposome Cyclodextrin Atomic force microscopy Vesicle General Physics and Astronomy Crystallography chemistry.chemical_compound chemistry Zeta potential Mica Physical and Theoretical Chemistry DNA Nuclear chemistry
Zdroj:	Chemical Physics Letters. 429:507-512
ISSN:	0009-2614
DOI:	10.1016/j.cplett.2006.08.043
Popis:	In the present study, anionic and pH-sensitive liposomes containing DNA were developed and characterized. These liposomes were obtained by binding the DNA with 6-monodeoxy-6-monoamine-β-cyclodextrin (Am-β-CD). This complex was encapsulated into the liposomes, which were characterized by encapsulation rate, diameter, zeta potential, and atomic force microscopy. The binding between Am-β-CD and the DNA was higher as of the +/− charge ratio. The amount of DNA encapsulated was approximately 10–14 μg/mL. The mean diameter and zeta potential were 186.0 nm and −56 mV, respectively. Liposomes which did not contain the complex were more prone to collapse over the mica surface. The vesicles containing the complex presented a narrower size distribution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7080d9f27c9f6e108f9bf34922001f53 https://doi.org/10.1016/j.cplett.2006.08.043 Zobrazit plný text záznamu Full Text from ScienceDirect