Effects of overexpressingp14 ARF on the apoptosis in human melanoma cells irradiated with γ-ray

Autor:	Wei Zhang, Dacheng He, Ping Gao, Huitu Liu, Lixia Peng
Rok vydání:	2003
Předmět:	Cytosol Multidisciplinary Tumor Suppressor ARF Cell cycle checkpoint medicine.anatomical_structure p14arf UVB-induced apoptosis Apoptosis medicine Mitochondrion Biology Nucleus Cell biology
Zdroj:	Chinese Science Bulletin. 48:1971-1975
ISSN:	1861-9541 1001-6538
DOI:	10.1007/bf03183989
Popis:	Tumor suppressor ARF can induce cell cycle arrest or apoptosis by activating p53. In order to explore the molecular mechanism of the induction of apoptosis byp14ARF, a human melanoma cell model overexpressingp14ARF was constructed. Present study indicated that in the cells overexpressingp14ARF, p53 was accumulated in nucleus while it dispersed in cytosol in the control cells. Irradiated with γ-ray, overexpressingp14ARF promoted the apoptosis of A375 cells, triggered Smac release from mitochondria to cytosol, and increased the expression of p53, Bax, Caspase-3, Caspase-9, p21cip1 and p27kip1. However, the protein level of Bcl-2 and phospho-ERK was down-regulated. These results suggested a possible mechanism of p14ARF in promotion of apoptosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::707ac9e4bfbe7dbe2e6bb065be2d002e https://doi.org/10.1007/bf03183989 Zobrazit plný text záznamu Full text from SpringerLink