Isolation and Structural Studies of Human Milk Oligosaccharides That Are Reactive with a Monoclonal Antibody MSW 1131
| Autor: | Shin-ichi Tate, Hiroshi Kitagawa, Mikiyo Takaoka, Toshisuke Kawasaki, Hiroshi Nakada, Ikuo Yamashina, Shigeyuki Fukui, Fuyuhiko Inagaki, Ikuo Funakoshi |
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| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Stereochemistry medicine.drug_class General Medicine Nuclear magnetic resonance spectroscopy Oligosaccharide Mass spectrometry Monoclonal antibody Biochemistry Affinity chromatography biology.protein medicine Molecule Carbohydrate conformation Antibody Molecular Biology |
| Zdroj: | The Journal of Biochemistry. 110:598-604 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a123626 |
| Popis: | We have determined the structures of six oligosaccharides isolated from human milk using a monoclonal antibody, MSW 113. The isolation involved affinity chromatography on a column of the immobilized monoclonal antibody and high-performance liquid chromatography. From the results of 500 and 600 mHz 1H NMR spectroscopy and fast atom bombardment-mass spectrometry their structures were deduced to be: [formula; see text] Two of these oligosaccharides, numbers 4 and 5, have not previously been described. All of them bound to MSW 113, but their reactivities are weaker than those of sialyl-Le(a) oligosaccharides. The results indicate that MSW 113 reacts with oligosaccharides with the mono- and disialyl-Le(a), and other sialyl type 1 structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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