| Autor: |
Myron B. Laver, S. Bert Litwin, F. Taketa, William F Skogen |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
Comparative Biochemistry and Physiology Part A: Physiology. 56:499-502 |
| ISSN: |
0300-9629 |
| DOI: |
10.1016/0300-9629(77)90275-4 |
| Popis: |
1. 1. Whole blood pH was monitored continuously during the generation of oxyhemoglobin dissociation curves with pH electrodes attached to a dissociation curve analyzer. 2. 2. Samples of whole blood drawn from non-smoking human adults showed a non-linear release of protons with a decrease in pH of 0.129 ± 0.008 in going from zero to 100% oxygen saturation. 3. 3. Oxygenation of human blood previously incubated with sodium cyanate produced a decrease in pH only 21.7% that of untreated cells in agreement with the known decrease in the Bohr effect caused by carbamylation of the α chain amino terminals of hemoglobin. 4. 4. The blood of various other mammalian species including pigs, rabbits, dogs and cats was also studied. Cat and rabbit samples demonstrated a significantly lower decrease in pH upon oxygenation than human, dog and pig blood. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
