Multi-omics reveals mechanisms of total resistance to extreme illumination of a desert alga
| Autor: | Yariv Brotman, Thiago Alexandre Moraes, Alexander Erban, Haim Treves, Ute Armbruster, Urszula Luzarowska, Jedrzej Szymanski, Mark Stitt, Joachim Kopka, Beata Siemiatkowska, Omer Murik, Stefan A. Rensing, Noe Fernandez-Pozo |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Chemistry Plant Science biology.organism_classification Photosynthesis Proteomics 01 natural sciences Redox 03 medical and health sciences Chlorella 030104 developmental biology Metabolomics Total inorganic carbon Thylakoid Lipidomics Biophysics 010606 plant biology & botany |
| Zdroj: | Nature Plants. 6:1031-1043 |
| ISSN: | 2055-0278 |
| Popis: | The unparalleled performance of Chlorella ohadii under irradiances of twice full sunlight underlines the gaps in our understanding of how the photosynthetic machinery operates, and what sets its upper functional limit. Rather than succumbing to photodamage under extreme irradiance, unique features of photosystem II function allow C. ohadii to maintain high rates of photosynthesis and growth, accompanied by major changes in composition and cellular structure. This remarkable resilience allowed us to investigate the systems response of photosynthesis and growth to extreme illumination in a metabolically active cell. Using redox proteomics, transcriptomics, metabolomics and lipidomics, we explored the cellular mechanisms that promote dissipation of excess redox energy, protein S-glutathionylation, inorganic carbon concentration, lipid and starch accumulation, and thylakoid stacking. C. ohadii possesses a readily available capacity to utilize a sudden excess of reducing power and carbon for growth and reserve formation, and post-translational redox regulation plays a pivotal role in this rapid response. Frequently the response in C. ohadii deviated from that of model species, reflecting its life history in desert sand crusts. Comparative global and case-specific analyses provided insights into the potential evolutionary role of effective reductant utilization in this extreme resistance of C. ohadii to extreme irradiation. The green alga, Chlorella ohadii, can survive under desert conditions of extreme irradiance. Redox proteomics, transcriptomics, metabolomics and lipidomics uncover how C. ohadii can absorb and use the sudden excesses of reducing power and carbon. |
| Databáze: | OpenAIRE |
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