Micro RNA-550a interferes with vitamin D metabolism in peripheral B cells of patients with diabetes
| Autor: | Ping-Chang Yang, Xiyun Guo, Jinggui He, Shao-Bo Yang, Zhi-Qiang Liu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Calcitriol Clinical Biochemistry 25-Hydroxyvitamin D3 1-alpha-hydroxylase Cell Biochemistry 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Internal medicine Diabetes mellitus medicine Vitamin D and neurology business.industry Cell Biology General Medicine medicine.disease 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Tumor necrosis factor alpha Calcifediol business medicine.drug |
| Zdroj: | Cell Biochemistry and Function. 34:640-646 |
| ISSN: | 0263-6484 |
| Popis: | The pathogenesis of diabetes is to be further investigated. Vitamin D3 (VitD3) can improve diabetes. Micro RNAs (miR) are involved in regulating cell activities. This study tests a hypothesis that miR-550a interferes with the metabolism of VitD3 in peripheral B cells. In this study, blood samples were collected from patients with diabetes and healthy persons. The B cells were isolated from the blood samples to be treated with tumor necrosis factor (TNF)-α. The B cells were then collected and analyzed for the expression of miR-550a and cyp27b1. The results showed that B cells from healthy subjects were capable of converting VitD metabolite calcidiol to calcitriol, which was impaired in B cells collected from diabetic patients. The diabetic patients showed lower bone mineral density than that in healthy subject. The miR-550a was negatively correlated with bone mineral density and the Levels of cyp27b1 in peripheral B cells of patients with diabetes. In vitro study showed that TNF-α increased miR-550a expression and inhibited the expression of cyp27b1 in B cells. miR-550a mediated the effects of TNF-α on inducing chromatin remodeling at the cyp27b1 gene locus. In conclusion, miR-550a mediates the TNF-α-induced suppression of cyp27b1 expression in peripheral B cells of patients with diabetes, which can be blocked by inhibition of miR-550a. |
| Databáze: | OpenAIRE |
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