Efficient Expression and Purification of Bombina maxima Trefoil Factors 2 Variant in Escherichia coli as Fusion Protein

Autor:	Tong Yi Sun
Rok vydání:	2014
Předmět:	Trefoil Factors biology Chemistry General Engineering Bombina maxima biology.organism_classification medicine.disease_cause Fusion protein Molecular biology law.invention law medicine Recombinant DNA Insoluble protein Wound healing agent Wound healing Escherichia coli
Zdroj:	Advanced Materials Research. :1187-1190
ISSN:	1662-8985
DOI:	10.4028/www.scientific.net/amr.926-930.1187
Popis:	Bm-TFF2 was isolated from Bombina maxima. The B.maxima Trefoil factors 2 Variant (vBm-TFF2) could be developed as a novel administered wound healing agent. The challenge is its preparation from refolding insoluble protein expressed in Escherichia coli. A recombinant vBm-TFF2 was overexpressed in E. coli cells as a fusion protein with bacterial N-utilizing substance A (NusA). The fusion protein NusA-Bm-TFF2 can promote the wound healing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::702429a67110335626ad2503149eb71f https://doi.org/10.4028/www.scientific.net/amr.926-930.1187 Zobrazit plný text záznamu