Flow-Injection Analysis Based on Extraction and Spectrophotometric Determination of Penicillins with Thiazine Dyes

Autor:	Irena Němcová, Petr Rychlovský, M. Havelcová
Rok vydání:	2009
Předmět:	Flow injection analysis Detection limit Chromatography Calibration curve Biochemistry (medical) Clinical Biochemistry Azure A Biochemistry Analytical Chemistry Standard curve chemistry.chemical_compound chemistry Thiazine Electrochemistry Pharmaceutics Spectroscopy Methylene blue
Zdroj:	Analytical Letters. 42:989-999
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032710902726088
Popis:	A new method for flow-injection analysis (FIA) for the determination of penicillins based on the extraction and spectrophotometric determination of ion associates with selected thiazine dyes (methylene blue, azure A, and azure B) is proposed. The reaction conditions (cdye = 2 × 10−4 mol l−1, cKCl = 1 mol l−1, pH ≅ 6, λ = 635 nm) were found. The factorial design has been carried out to determine the optimum flow conditions. A wide linear dynamic range of calibration curves (5.1–700 µg ml−1 for penicillin V with all dyes, R = 0.9985) and good repeatability (e.g., relative standard deviation [RSD] = 4.6–0.6% in this concentration range for the reaction with azure B) were found. The detection limit for penicillin V is 1.5 µg ml−1, and the determination limit is 5.1 µg ml−1. The maximum analysis rate is 35 samples per h. The practical samples of pharmaceutics were tested. There are no interferences from the additives in pharmaceutics.
Databáze:	OpenAIRE
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