Traumatic muscle injury promotes aberrant fibro-adipogenic progenitor cell population, leading to fibrosis and impaired muscle stem cell function

Autor: Nyssa Morgan, Hongmanlin Zhang, Shannon Anderson, Young Jang
Rok vydání: 2023
Předmět:
Zdroj: Physiology. 38
ISSN: 1548-9221
1548-9213
DOI: 10.1152/physiol.2023.38.s1.5734945
Popis: Skeletal muscle has an exceptional regenerative capacity that relies on the temporal activation and regulation of immune cells, fibro-adipogenic progenitors (FAPs), and muscle stem cells (MuSCs). However, following traumatic injury to skeletal muscle, such as in extensive muscle deficits caused by injury or surgery, also known as volumetric muscle loss (VML), this coordinated regenerative response is diminished, leading to fibrosis, inflammation, and chronic functional deficiencies. The goal of this study was to compare the temporal response and phenotype of key cell populations, including macrophages, FAPs, and MuSCs following critical and sub-critical VML injuries in the mouse quadriceps. We hypothesize that traumatic injury, specifically VML, drives cell phenotypic variability amongst these cell populations. Unilateral VML injuries were used to quantify cell type distribution via flow cytometry at 1, 3, and 7 days post-VML (pVML, n=4 per injury size and time point). At day 7 pVML, anti-inflammatory M2 macrophages, FAPs, and MuSCs were all present in significantly elevated numbers per tissue volume in critical (3-mm) injuries compared to sub-critical (2-mm) injuries (p Funding: This work was supported by the National Institute of Health under award number R01AG072309, R01AR071708, and Department of Defense under award number W81XWH-20-1-0336. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Databáze: OpenAIRE
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