IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS
| Autor: | N. Shubhashini, H B Swetha, Vinaya Kumar R, Nithin Kumar Shetty, B S Srikanth Choudhary, Annapoorna Kini |
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| Rok vydání: | 2020 |
| Předmět: |
biology
Antimicrobial efficacy 0206 medical engineering 030206 dentistry 02 engineering and technology Contamination biology.organism_classification 020601 biomedical engineering In vitro Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Sodium hypochlorite Toluidine Methylene blue Nuclear chemistry |
| Zdroj: | GLOBAL JOURNAL FOR RESEARCH ANALYSIS. :148-151 |
| Popis: | AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue. |
| Databáze: | OpenAIRE |
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