How to sample a seizure plant: the role of the visualization spatial distribution analysis of Lophophora williamsii as an example
| Autor: | Jiaman Lin, Keming Yun, Qiran Sun, Ping Xiang, Lina Wu, Shuo Yang, Junling Dun, Shanlin Fu, Hang Chen |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Forensic Sciences Research. |
| ISSN: | 2471-1411 2096-1790 |
| DOI: | 10.1093/fsr/owad014 |
| Popis: | How to sample a seizure plant:the role of visualization spatial distribution analysis of Lophophora williamsii as an exampleAbstract: Natural compounds in plants are often unevenly distributed, and determining the best sampling locations to obtain the most representative results is technically challenging. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can provide the basis for formulating sampling guideline. For a succulent plant sample, ensuring the authenticity and in situ nature of the spatial distribution analysis results during MSI analysis also needs to be thoroughly considered. In this study, we developed a well-established and reliable MALDI-MSI method based on preservation methods, slice conditions, auxiliary matrices, and MALDI parameters to detect and visualize the spatial distribution of mescaline in situ in L. williamsii. The MALDI-MSI results were validated using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Low-temperature storage at −80°C and drying of ‘bookmarks’ were the appropriate storage methods for succulent plant samples and their flower samples, and cutting into 40μm thick sections at −20°C using gelatin as the embedding medium is the appropriate sectioning method. The use of DCTB (trans-2-[3-(4-tert-butylphenyl)-2-methyl-2-propenylidene]malononitrile) as an auxiliary matrix and a laser intensity of 45 are favorable MALDI parameter conditions for mescaline analysis. The region of interest (ROI) semi-quantitative analysis revealed that mescaline is concentrated in the epidermal tissues of L. williamsii as well as in the meristematic tissues of the crown. The study findings not only help to provide a basis for determining the best sampling locations for mescaline in L. williamsii, but they also provide a reference for the optimization of storage and preparation conditions for raw plant organs before MALDI detection. |
| Databáze: | OpenAIRE |
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